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anti bactin  (Boster Bio)


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    Structured Review

    Boster Bio anti bactin
    Anti Bactin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 338 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+beta+actin+actb+antibody/Anti-Beta-actin+ACTB+Antibody/pm41990973-117-15-16
    Average 93 stars, based on 338 article reviews
    anti bactin - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Effects of intermittent hypoxia on adipose-derived mesenchymal stem cells in protecting alveolar type II cells from injury
    Article Snippet: Samples were separated on SDS-PAGE gels (Epizyme, Shanghai, China) and transferred to polyvinylidene fluoride (PVDF) membranes. .. Membranes were blocked at room temperature for 20 min with Protein Free Rapid Blocking Buffer (Epizyme, Shanghai, China) and then incubated with primary antibodies: Bax antibody and Bcl-2 antibody (1:2000, 1:1000, Wanleibio, Shenyang, China), Cleaved Caspase 3 Polyclonal antibody and Caspase 3/P17/P19 Polyclonal antibody (1:1000, 1:2000, Proteintech, Wuhan, China), Recombinant Anti-HIF-1 alpha antibody (Rabbit mAb) (1:500, Servicebio, Wuhan, China) and Anti-Beta Actin/ACTB Antibody (1:8000, Boster Biological Technology, Wuhan, China) overnight were incubated at 4°C. .. PVDF membranes were then incubated with HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H + L) (1:10000, Boster Biological Technology, Wuhan, China) for 1 h at room temperature.

    Incubation:

    Article Title: Effects of intermittent hypoxia on adipose-derived mesenchymal stem cells in protecting alveolar type II cells from injury
    Article Snippet: Samples were separated on SDS-PAGE gels (Epizyme, Shanghai, China) and transferred to polyvinylidene fluoride (PVDF) membranes. .. Membranes were blocked at room temperature for 20 min with Protein Free Rapid Blocking Buffer (Epizyme, Shanghai, China) and then incubated with primary antibodies: Bax antibody and Bcl-2 antibody (1:2000, 1:1000, Wanleibio, Shenyang, China), Cleaved Caspase 3 Polyclonal antibody and Caspase 3/P17/P19 Polyclonal antibody (1:1000, 1:2000, Proteintech, Wuhan, China), Recombinant Anti-HIF-1 alpha antibody (Rabbit mAb) (1:500, Servicebio, Wuhan, China) and Anti-Beta Actin/ACTB Antibody (1:8000, Boster Biological Technology, Wuhan, China) overnight were incubated at 4°C. .. PVDF membranes were then incubated with HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H + L) (1:10000, Boster Biological Technology, Wuhan, China) for 1 h at room temperature.

    Recombinant:

    Article Title: Effects of intermittent hypoxia on adipose-derived mesenchymal stem cells in protecting alveolar type II cells from injury
    Article Snippet: Samples were separated on SDS-PAGE gels (Epizyme, Shanghai, China) and transferred to polyvinylidene fluoride (PVDF) membranes. .. Membranes were blocked at room temperature for 20 min with Protein Free Rapid Blocking Buffer (Epizyme, Shanghai, China) and then incubated with primary antibodies: Bax antibody and Bcl-2 antibody (1:2000, 1:1000, Wanleibio, Shenyang, China), Cleaved Caspase 3 Polyclonal antibody and Caspase 3/P17/P19 Polyclonal antibody (1:1000, 1:2000, Proteintech, Wuhan, China), Recombinant Anti-HIF-1 alpha antibody (Rabbit mAb) (1:500, Servicebio, Wuhan, China) and Anti-Beta Actin/ACTB Antibody (1:8000, Boster Biological Technology, Wuhan, China) overnight were incubated at 4°C. .. PVDF membranes were then incubated with HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H + L) (1:10000, Boster Biological Technology, Wuhan, China) for 1 h at room temperature.



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    Image Search Results


    Journal: Frontiers in Physiology

    Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

    doi: 10.3389/fphys.2026.1781613

    Figure Lengend Snippet: Primer sequences for the genes observed in qRT-PCR.

    Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

    Techniques:

    Effects of HH on the BBB integrity in mouse hippocampus. (A) Representative images of EB extravasation in the hippocampus. (B) Quantitative analysis of EB leakage. (C) Representative western blots of ZO-1 and occludin. Quantitative analysis of the ZO-1/β-actin (D) and occludin/β-actin (E) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For EB, n = 6 per group; for Western blot analysis, n = 3 per group, ** p < 0.01 vs. Con group.

    Journal: Frontiers in Physiology

    Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

    doi: 10.3389/fphys.2026.1781613

    Figure Lengend Snippet: Effects of HH on the BBB integrity in mouse hippocampus. (A) Representative images of EB extravasation in the hippocampus. (B) Quantitative analysis of EB leakage. (C) Representative western blots of ZO-1 and occludin. Quantitative analysis of the ZO-1/β-actin (D) and occludin/β-actin (E) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For EB, n = 6 per group; for Western blot analysis, n = 3 per group, ** p < 0.01 vs. Con group.

    Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

    Techniques: Western Blot

    Effects of HH on the expression of genes and proteins related to PI3K/AKT signaling pathway in mouse hippocampus. (A) The relative mRNA expression levels of Kdr , Spp1 , Vwf , and Vegfa screened via qRT-PCR. (B) Representative western blots of p-PI3K, PI3K, p-AKT, and AKT. Quantitative analysis of the p-PI3K/PI3K (C) and p-AKT/AKT (D) ratios. (E) Representative western blots of Nrf2, HO-1, p-NF-κB, and NF-κB. Quantitative analysis of the Nrf2/β-actin (F) , HO-1/β-actin (G) , and p-NF-κB/NF-κB (H) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For qRT-PCR, n = 6 per group; for Western blot analysis, n = 3 per group, * p < 0.05, ** p < 0.01 vs. Con group.

    Journal: Frontiers in Physiology

    Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

    doi: 10.3389/fphys.2026.1781613

    Figure Lengend Snippet: Effects of HH on the expression of genes and proteins related to PI3K/AKT signaling pathway in mouse hippocampus. (A) The relative mRNA expression levels of Kdr , Spp1 , Vwf , and Vegfa screened via qRT-PCR. (B) Representative western blots of p-PI3K, PI3K, p-AKT, and AKT. Quantitative analysis of the p-PI3K/PI3K (C) and p-AKT/AKT (D) ratios. (E) Representative western blots of Nrf2, HO-1, p-NF-κB, and NF-κB. Quantitative analysis of the Nrf2/β-actin (F) , HO-1/β-actin (G) , and p-NF-κB/NF-κB (H) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For qRT-PCR, n = 6 per group; for Western blot analysis, n = 3 per group, * p < 0.05, ** p < 0.01 vs. Con group.

    Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot